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Acetoacetate（AcAc）Content Assay Kit

Cat.No：BC5075 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Acetoacetate（AcAc）Content Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Acetoacetic acid (AcAc) is one of the important components of ketone body. It is about 20% of the total ketone body in normal people. It is a strong organic acid produced by oxidation of fatty acids. Normal content of acetoacetic acid is harmless to human body. In diabetic patients, the amount of acetoacetic acid is accumulated due to the decrease of carbohydrate use or starvation due to the metabolic disorder of sugar. Acetoacetic acid can be converted into acetone as well as acetone β- Hydroxybutyric acid.

Measurement Principle：

At pH 7.0 and 37℃, β-Hydroxybutyrate dehydrogenase (HBDH) reduced AcAc to 3-hydroxybutyrate or decarboxylated to acetone, and NADH was oxidized to NAD+. In the presence of 1-mPMS, WST-1 can react with NADH to produce water-soluble formazan with a characteristic absorption peak at 450nm. The content of AcAc can be calculated by detecting the wavelength change at 450 nm.

Self Provided：

Spectrophotometer/microplate reader, desk centrifuge, pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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