Ketone Body Content Assay Kit
Cat.No：BC5065 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme I Series > Ketone Body Content Assay Kit
Product Information
| Name | Ketone Body Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Ketone bodies are intermediate products of fatty acid oxidative decomposition in liver. It includes Acetoacetic acid (AcAc) and β- Hydroxybutyric acid (BOH) and acetone. The amount of acetone in ketone body is very small, and it is absorbed immediately. AcAc and BOH is oxidized in extrahepatic tissue through blood flow. The citric acid cycle provides more energy for those tissues, such as bone, myocardium and renal cortex.
Measurement Principle：
At pH 7.0 and 37℃, β- Hydroxybutyrate dehydrogenase (HBDH) catalyzes the dehydrogenation of BOH to produce phthalic acid, and NAD+ is reduced to NADH. At pH 8.8 and 37℃, HBDH reduced AcAc to 3-hydroxybutyrate or decarboxylated to acetone, and NADH was oxidized to NAD+. NADPH has a characteristic absorption peak at 340nm. The content of BOH and AcAc can be calculated by detecting the change of absorbance at 340nm. Then the content of ketone body in the sample can be calculated.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, pipette, micro quartz cuvette/96 well UV flat -bottom plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Hepatic ELOVL3 is dispensable for lipid metabolism in mice
Click to check >>Author：Zhenbang Qin, Ping Wang, Weiwen Chen, Jue-Rui Wang, Xianhua Ma, Hai Zhang, Weiping J. Zhang, Chunchun Wei
IF：3.3220
Publish_to：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：37030067
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