Ultrafiltration centrifugal tube

Material: polyethylene cap,polyethylene liner,polypropylene tube,regenerated cellulose membrane,regenerated cellulose support,styrene-butadiene housing

Quality Level: 400

Sterility: non-sterile

Product line: Amicon®

Manufacturer/tradename:Amicon® Ultra

Parameter: 4 mL sample volume

Technique(s): protein extraction: suitable, protein purification: suitable

L: 124mm (4.88in.)

Diam.: 17.3mm

Filtration area: 3cm2

Volume: 4mL

Pore size: 100kDa NMWCO

Shipped in: ambient

General description:

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156kDa) at 1 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15mL filters deliver the highest recovery with short spin times.

Application:

1 Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.

2 Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.

3 Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.

4 Concentration of dilute or pre-purified proteins from column eluents.

5 Desalting, buffer exchange and protein dialysis.

6 Size exclusion and protein fractionation.

Features and Benefits:

1 Ultracel® regenerated cellulose membrane with 50kDa NMWCO delivers retentate recovery of greater than 90%.

2 Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).

3 Heat-sealed membrane minimizes downstream extractables.

4 100% integrity tested for reliable performance.

5 Convenient sample monitoring with translucent housing and volume gradations.

6 Direct pipette sample access eliminates processing step to recover concentrate.

7 High concentration factors of 80–100X.

Color Code: Clear with pink cap

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.