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My CartReactivity：Arabidopsis thaliana
Application： WB,IHC
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Mouse
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| Name | Anti-ABI4 Monoclonal Antibody for Plant |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1:2000. IHC 1:50-1:200 |
| Target | ABI4 |
| Background | Transcription regulator that probably binds to the GCC-box pathogenesis-related promoter element. Binds also to the S-box (5'-CACTTCCA-3') photosynthesis-associated nuclear genes-related (PhANGs-related) promoter element, and thus acts as a transcription inhibitor. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. May have a function in the deetiolation process. Confers sensitivity to abscisic acid (ABA), and regulates the ABA signaling pathway during seed germination, upon nitrate-mediated lateral root inhibition, in hexokinase-dependent sugar responses (including feed-back regulation of photosynthesis and mobilization of storage lipid during germination), and in response to osmotic stress mediated by NaCl, KCl or mannitol. Plays a role in sucrose sensing or signaling, especially at low fluence far red light. Also involved in plant response to glucose treatment, especially at low concentration and in young seedlings. Required for the trehalose-mediated root inhibition and starch accumulation in cotyledons, probably by inhibiting starch breakdown. However, seems to not be involved in sugar-mediated senescence. Required for the ABA-dependent beta-amino-butyric acid (BABA) signaling pathway. BABA primes ABA synthesis and promotes resistance to drought and salt, and leads to a prime callose accumulation that confers resistance against necrotrophic pathogens such as A.brassicicola and P.cucumerina. Seems to be involved in resistance to S.sclerotiorum probably by regulating the ABA-mediated stomatal closure apparently by antagonistic interaction with oxalate. Negative regulator of low water potential-induced Pro accumulation whose effect is decreased by high levels of sugar. |
| Swiss Prot | A0MES8 |
| Gene ID | 818614 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein derived from ABI4 in Arabidopsis thaliana AT2G40220 |
| Calculated MW | 35.67 kDa |
| Observed MW | 32 kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-1:2000. IHC 1:50-1:200. |
| Specification | 50ul 100ul |
| Reactivity | Arabidopsis thaliana |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
25μg total protein from Arabidopsis thaliana. Western blot analysis with anti-ABI4 mAb for plant diluted at 1:1000.
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