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Serum Amylase（AMY）Activity Assay Kit

BC5050 Manual

Cat.No：BC5050 Solarbio

Storage：Store at 2-8℃，6 months

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Product Information

Name	Serum Amylase（AMY）Activity Assay Kit
Detection Method	Spectrophotometer
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	50T/24S

Determination of Significance：

Serum amylase (AMY) belongs to α-amylase, which hydrolyzes α-1,4 glycosidic bonds inside polysaccharide molecules in a random manner to generate a mixture of oligosaccharides, maltose and glucose. AMY is mainly secreted by the salivary glands and pancreas, and a small amount of it is secreted by organs such as the proximal duodenum, lungs, uterus, and breast during lactation.

Measurement Principle：

Amylase catalyzes the hydrolysis of α-1,4 glycosidic bonds in starch molecules to produce glucose, maltose, dextrin, etc. Iodine can be combined with starch that is not hydrolyzed by amylase to form a complex with a characteristic absorption peak at 570 nm. The depth can calculate the unit of amylase activity. α-amylase is acid-resistant and β-amylase is heat-resistant. According to the above characteristics, the activity of another amylase can be measured by passivating one of them.

Self Provided：

Spectrophotometer, water bath/ incubator, desktop centrifuge, transferpettor, mortar/homogenizer, 1 mL glass cuvette, mortar, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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