Mouse Neutrophil Elastase ELISA Kit
Cat.No：SEKM-0237 Solarbio
Application： ELISA
Reactivity：Mouse
Sensitivity：25 pg/ml
Storage：2-8℃，6 months
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HIF
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Mouse Neutrophil Elastase ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Neutrophil Elastase/ELA2 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 50-3200 pg/ml |
| Sensitivity | 25 pg/ml |
| Specificity | Do not react with Mouse SerpinA1a，SerpinA1c，SLPI， Human ELA2 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q3UP87 |
| Gene ID | 50701 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠ELA2单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠ELA2会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠ELA2抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠ELA2发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠ELA2，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠ELA2浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠ELA2的浓度。 |
| Background | Neutrophil Elastase (ELA2)，also known as polymorphonuclear leukocyte elastase，is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes，ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema，cystic fibrosis，the adult respiratory distress syndrome (ARDS)，rheumatoid arthritis，tumor invasion and infectious diseases. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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