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QuickCut restriction endonuclease AscI

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Cat.No：Q2010 Solarbio

Storage：Store at -20℃，1 year.

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Product Information

Name	QuickCut restriction endonuclease AscI
Storage	Store at -20℃，1 year.
Unit	Piece
Specification	50Rxns

Fast endonuclides are a series of restriction endonuclides that have been genetically engineered to precisely cut DNA in 5 to 15 minutes, and are suitable for rapid enzyme cutting of plasmid DNA, PCR products or genomic DNA. Fast endonuclease has the following characteristics: it can be completed in 5~15 minutes; Sharing one digestion Buffer greatly simplifies the digestion reaction system. Good redundancy of enzyme activity, easy to cope with substrate excess or difficult template enzyme cutting. In addition, dephosphorylation and ligation reagents are 100% active in the digestion Buffer, supporting a tubeization reaction and improving the enzyme-modified-ligation experience.

REF: MD030

5 '... G G/ C G C G C C... 3 '

3 '... C C G C G C /G G... 5 '

Fast enzyme that can complete the reaction in 5-15 minutes

Reaction conditions: 10× FastCut Buffer&10× FastCut Color Buffer, 37℃. Thermal inactivation: 80℃for 20 minutes

Methylation sensitivity: Influenced by CpG methylation, shear may be affected when sequence overlaps

Asterisk activity: 1 hour incubation did not show asterisk activity, 16 hours may appear asterisk activity

Isomerase: PalAI, SgsI

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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