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My CartCAS：208260-29-1
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to yellow Solid
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| CAS | 208260-29-1 |
| Name | ZM-336372 |
| Molecular Formula | C23H23N3O3 |
| Molecular Weight | 389.45 |
| Solubility | Soluble in DMSO ≥5mg/mL;Soluble in Water ＜ 1mg/mL |
| Purity | ≥98% |
| Appearance | White to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD02683971 |
| SMILES | O=C(NC1=CC=C(C)C(NC(C2=CC=C(O)C=C2)=O)=C1)C3=CC=CC(N(C)C)=C3 |
| InChIKey | PYEFPDQFAZNXLI-UHFFFAOYSA-N |
| InChI | InChI=1S/C23H23N3O3/c1-15-7-10-18(24-23(29)17-5-4-6-19(13-17)26(2)3)14-21(15)25-22(28)16-8-11-20(27)12-9-16/h4-14,27H,1-3H3,(H,24,29)(H,25,28) |
| PubChem CID | 5730 |
| Target Point | C-Raf |
| Passage | MAPK |
| Background | ZM 336372 is a potent inhibitor of protein kinase c-Raf. |
| Biological Activity | ZM 336372 是一种有效的蛋白激酶 c-Raf 抑制剂。在含有 0.1 mM ATP 的情况下，IC 50 值为 0.07 μM。[1-2] |
| In Vitro | ZM 336372是人类c-Raf的有效抑制剂。在标准测定中IC 50值为0.07μM，其含有0.1mM ATP。 IC50在0.025mM ATP下降至0.01μM，在2.5mM ATP下降至0.9μM，表明ZM 336372是ATP的竞争性抑制剂。 ZM 336372比B-Raf更有效地抑制c-Raf十倍[1]。 ZM336372的细胞增殖分析。将H727和BON的3，4-(4，5-二甲基噻唑-2-基)-2，5-二苯基四唑溴化物生长测定分别作为对照，DMSO和100μMZM336372处理至第16天和第10天。与对照相比，在药物存在下H727和BON细胞增殖都被抑制。用ZM336372处理的H727细胞生长被抑制，而对照处理在第6天具有显着更多的生长，持续长达16天。在BON细胞中也观察到类似的反应，因为生长抑制早在第4天就发生并且维持到第10天[2]。 |
| Cell Experiment | 使用3，4-(4，5-二甲基噻唑-2-基)-2，5-二苯基四唑溴化物测量用ZM336372处理后H727和BON细胞的增殖。将细胞用胰蛋白酶消化并一式三份铺板到24孔板中并使其粘附过夜。然后，用100μMZM336372或DMSO(2％)处理细胞并孵育。使用新的治疗方法每2天更换一次培养基。在每个时间点，在向培养的细胞中加入3，4-(4，5-二甲基噻唑-2-基)-2，5-二苯基溴化四唑试剂后，分析细胞生长速率。使用分光光度计在540nm的波长下测定吸光度[2]。 |
| Data Literature Source | [1]. Hall-Jackson CA，et al. Paradoxical activation of Raf by a novel Raf inhibitor. Chem Biol. 1999 Aug;6(8):559-68. [2]. Van Gompel JJ，et al. ZM336372，a Raf-1 activator，suppresses growth and neuroendocrine hormone levels in carcinoid tumor cells. Mol Cancer Ther. 2005 Jun;4(6):910-7. |
| Unit | Bottle |
| Specification | 5mg 10mg |
ZM 336372 是一种有效的蛋白激酶 c-Raf 抑制剂。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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