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Application： ELISA
Reactivity：Porcine
Sensitivity：15 pg/ml
Storage：2-8℃，6 months
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| Name | Porcine IL-16 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-16 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000 pg/ml |
| Sensitivity | 15 pg/ml |
| Specificity | Do not react with IL-2，IL-4，IL-5，IL-6，IFN-γ，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR4，TNF-α etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪IL-16单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的猪IL-16会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗猪IL-16抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的猪IL-16发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的猪IL-16，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的猪IL-16浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中猪IL-16的浓度。 |
| Background | Interleukin-16 (IL-16) is a cytokine that is encoded by the IL16 gene.The cytokine is a pleiotropic cytokine that functions as a chemoattractant，a modulator of T cell activation，and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. IL-16 undergoes proteolytic processing，which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted Cterminal peptide，while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of IL-16. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. IL-16 is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. IL-16 was originally described as a factor that could attract activated T cells in humans，it was previously called lymphocyte chemoattractant factor (LCF). Since then，this interleukin has been shown to recruit and activate many other cells expressing the CD4 molecule，including monocytes，eosinophils，and dendritic cells.The structure of IL-16 was determined following its cloning in 1994.This cytokine is produced as a precursor peptide (pro-IL-16) that requires processing by an enzyme called caspase-3 to become active. CD4 is the cell signaling receptor for mature IL-16 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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