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Application： ELISA
Reactivity：Porcine
Sensitivity：3pg/mL
Storage：2-8℃，6 months
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| Name | Porcine IL-9 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-9 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.625-1000pg/mL |
| Sensitivity | 3pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h15min |
| Background | interleukins. IL-9 is produced by variety of cells like mast cells，NKT cells，Th2，Th17，Treg，ILC2，and Th9 cells in different amounts. Among them，Th9 cells are regarded as the major CD4+ T cells that produce IL-9. Il-9 is secreted by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells. Interleukin 33 (IL-33) induces IL-9 expression and secretion in T cells，which was confirmed by the results obtained in mice by using Human in vitro system. Whereas the report of others confirms that TGF-β is an essential factor for IL-9 induction. For the first time (Lars Blom，Britta C. Poulsen，Bettina M. Jensen，Anker Hansen and Lars K. Poulsen published a journal online in 2011 Jul 6)，indicating that TGF-β may be important for production of IL-9 but it is not only the definite requirement for IL-9 induction，since cultures with IL-33 without TGF-β have noticeably increased secretion of IL-9，suggesting an important role of IL-33，even though that the effect was not found significant on the gene level. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R)，which activates different signal transducer and activator (STAT) proteins namely STAT1，STAT3 and STAT5 and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. Interleukin-9 has also shown to inhibit melanoma growth in mice.Additionally，it gives rise to the multiplication of hematologic neoplasias and also Hodgkin's lymphoma in humans but IL-9 also has antitumor properties in solid tumors，for example melanoma. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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