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Creatinine（Cr）Content Assay Kit

BC4915Manual

Cat.No：BC4915 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Creatinine（Cr）Content Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/96S

Determination of Significance：

Creatinine (Cre), the chemical formula is C4H7N3O, is a product of muscle metabolism in the human body, which is mainly excreted by glomerular filtration. Serum creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of muscle metabolism in the body.

Measurement Principle：

The creatinine in the sample is hydrolyzed to creatine under the catalysis of creatinine enzyme. Under the catalysis of creatinase, creatine hydrolyzes to creatine and urea. Sarcosine is oxidized to hydrogen peroxide under the catalysis of sarcosine oxidase. Peroxidase catalyzed the oxidation of 4-aminoantipyrine coupling phenol by hydrogen peroxide to form colored compounds with characteristic absorption peak at 505nm.

Self Provided：

Spectrophotometer/microplate reader, low tempareture centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water, ultrasonic crusher.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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