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Application： ELISA
Reactivity：Mouse
Sensitivity：62.5 pg/ml
Storage：2-8℃，6 months
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| Name | Mouse BMP6 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | BMP6 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 125-8000 pg/ml |
| Sensitivity | 62.5 pg/ml |
| Specificity | Do not react with Mouse ApoAI，BMP1，BMP2，BMP3，BMP4，HGF，HSP27，IFN，IL-1β，IL-1RA，IL-2，IL-5，IL-6，IL8，IL-10，IL-12，IL-13，IL-15，IL-17C，IL-23，MMP-2，MMP-9，sIL-2R，sIL-6R，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR3，TNF-α，TNFRI，TNFRII，VEGF. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | P20722 |
| Gene ID | 12161 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠BMP-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠BMP-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠BMP-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠BMP-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠BMP-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠BMP-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠BMP-6的浓度。 |
| Background | Bone morphogenetic protein 6 (BMP6) is a protein that in humans is encoded by the BMP6 gene. [1][2] The protein encoded by this gene is a member of the TGFβ superfamily. Bone morphogenetic proteins (BMPs) are known for their ability to induce the growth of bone and cartilage. BMP6 is able to induce all osteogenic markers in mesenchymal stem cells. The BMPs are a family of secreted signaling molecules that can induce ectopic bone growth. BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis，the BMP encoded by this gene has a proposed role in early development. In addition，the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. As of April 2009，an additional function of BMP6 has been identified as described in Nature Genetics April; 41 [4]:386-8. BMP6 is the key regulator of hepcidin，the small peptide secreted by the liver which is the major regulator of iron metabolism in mammals. BMP6 is expressed in normal and atherosclerotic smooth muscle cells，[3] normal and malignant prostatic tissue，[4] healthy and osteoarthritic human articular chondrocytes and can stimulate matrix synthesis in vitro.[5] Bone morphogenetic proteins 2 and 6，expressed in arthritic synovium，are regulated by proinflammatory cytokines and differentially modulate fibroblast-like synoviocyte apoptosis.[6] |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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