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Application： ELISA
Reactivity：Fish
Sensitivity：46 pg/ml
Storage：2-8℃，6 months
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| Name | Fish GH ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | GH |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Fish |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 93.75-6000 pg/ml |
| Sensitivity | 46 pg/ml |
| Specificity | The cross-reaction rate is less than 10%. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗鱼GH单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的鱼GH会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗鱼GH抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的鱼GH发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的鱼GH，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的鱼GH浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中鱼GH的浓度。 |
| Background | Fish GH is a single chain protein hormone secreted by eosinophils in the anterior lobe of the pituitary gland，which consists of 173 to 188 amino acids and has a molecular weight of 20000 to 22000 Daltons. Fish GH has some homology with other vertebrates GH in terms of molecular weight，amino acid composition and sequence. GH is a key regulatory factor in the growth and development of fish body. GH can act on almost all tissues of the body，stimulate tissue development and increase the size and number of somatic cells. In addition，also has the regulation of fish metabolism，enhance immunity，promote cell division. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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