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Application：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：4.8 pg/ml
Storage：2-8℃，6 months
Gene ID：21938
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| Name | Mouse TNFR2/TNFRSF1B ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | TNFR2/TNFRSF1B |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 9.375-600 pg/ml |
| Sensitivity | 4.8 pg/ml |
| Specificity | Do not react with C10，Eotaxin，G-CSF，GM-CSF，IFN-γ，IL-1α，IL-1β，IL-1ra，IL-2，IL-3，IL-4，IL-5，IL-6，IL-7，IL-9，IL-10，IL-10R，IL-12，IL-13，IL-17，IL-18，JE/MCP-1，KC，Leptin，LIF，M-CSF，MIP-1α，MIP-1β，MIP-2，OSM，SCF，TNF-α，TNFRI，Tpo，VEGF etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P25119 |
| Gene ID | 21938 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠TNFR2/TNFRSF1B单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠TNFR2/TNFRSF1B会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗小鼠TNFR2/TNFRSF1B抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠TNFR2/TNFRSF1B发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠TNFR2/TNFRSF1B，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠TNFR2/TNFRSF1B浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠TNFR2/TNFRSF1B的浓度。 |
| Background | Tumor necrosis factor receptor superfamily，member 1B (TNFRSF1B)，also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen，is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins，c-IAP1 and c-IAP2，which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes，its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily，member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis，which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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