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My Cart> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Cytoskeleton > Mdivi-1CAS：338967-87-6
Molecular Formula：C15H10Cl2N2O2S
Molecular Weight：353.22
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to brown Solid
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| CAS | 338967-87-6 |
| Name | Mdivi-1 |
| Molecular Formula | C15H10Cl2N2O2S |
| Molecular Weight | 353.22 |
| Solubility | Soluble in DMSO |
| Purity | ≥98% |
| Appearance | White to brown Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD00974506 |
| SMILES | O=C1N(C2=C(Cl)C=C(Cl)C(OC)=C2)C(S)=NC3=C1C=CC=C3 |
| Target Point | Dynamin |
| Passage | Cytoskeleton |
| Background | Mdivi-1 is a potent mitochondrial division/mitophagy inhibitor that inhibits DRP1 (motor protein-associated GTPase) and Dynamin I (Dnm1) activity. |
| Biological Activity | Mdivi-1是选择性的发动蛋白相关蛋白1 (Drp1) 抑制剂。Mdivi-1以剂量依赖性方式抑制Dnm1 GTP酶活性，估计EC50为1-10μM。[1-4] |
| In Vitro | 用mdivi-1处理的细胞显示细胞色素c释放减少，细胞凋亡诱导后表面磷脂酰丝氨酸暴露率降低，与细胞凋亡的抑制一致，以及之前的研究使用其他策略来破坏DRP1活性[2]。 Mdivi-1导致缺血性视网膜中的凋亡细胞死亡[3]。 |
| In Vivo | 线粒体分裂DRP，Dnm1，是体内mdivi-1的靶标。 Mdivi-1定量阻断GMPPCP依赖性Dnm1自组装，其浓度范围与其对体内线粒体分裂的影响相似[1]。 Mdivi-1(50 mg/kg，ip)显著降低正常小鼠视网膜中GFAP蛋白的表达[3]。 |
| Cell Experiment | YPGlycerol板顶部装有10mL含有1％低熔点琼脂和75μMmdivi-1的YP甘油，12小时后使用48孔钉扎装置点样细胞。钉住细胞后，将板在24℃或37℃温育，并使用Eagle Eye II成像系统成像。 |
| Kinase Experiment | 所有GTP酶测定反应均在200μL体积中开始，其中将150μL置于96孔板的孔中。通过读取反应的A340监测的NADH的消耗，使用SpectraMAX 250 96孔板读数器每20秒测量总共40分钟。将分光光度数据转移至Excel，并将测得的NADH稳态耗尽随时间转化为蛋白质活性。 |
| Data Literature Source | [1]. Cassidy-Stone A，et al. Chemical inhibition of the mitochondrial division dynamin reveals its role in Bax/Bak-dependent mitochondrial outer membrane permeabilization. Dev Cell. 2008 Feb;14(2):193-204. [2]. Tanaka A，et al. A chemical inhibitor of DRP1 uncouples mitochondrial fission and apoptosis. Mol Cell. 2008 Feb 29;29(4):409-10. [3]. Park SW，et al. A selective inhibitor of drp1，mdivi-1，increases retinal ganglion cell survival in acute ischemic mouse retina. Invest Ophthalmol Vis Sci. 2011 Apr 27;52(5):2837-43. [4]. Zhou K，et al. RIP1-RIP3-DRP1 pathway regulates NLRP3 inflammasome activation following subarachnoid hemorrhage. Exp Neurol. 2017 Sep;295:116-124. |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 20mg |
Mdivi-1是一个具有选择性和细胞穿膜性的线粒体分裂抑制剂。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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