{{cart_num}}
My Cart
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：3.9 pg/ml
Storage：2-8℃，6 months
Gene ID：50781
Qty：
Size：
| Name | Mouse Dkk-3 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Dkk-3 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 7.81-500 pg/ml |
| Sensitivity | 3.9 pg/ml |
| Specificity | In direct ELISA, cross-reactivity with recombinant Human (rh)DKK3 is about 20% and less than 1% with rhDKK1. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q9QUN9 |
| Gene ID | 50781 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠DKK3单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠DKK3会与酶标板上的包被抗体充分结合；洗板后加入辣根过氧化物酶标记的抗小鼠DKK3抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠DKK3发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠DKK3，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠DKK3浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠DKK3的浓度。 |
| Background | DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1，DKK2，DKK3 and DKK4. It is a secreted protein，and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
