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My Cart> Antibodies > Loading Control Antibodies > Nucleus > Anti-PARP1 Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application： IF,ELISA,FCM,IHC,WB,IP
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
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| Name | Anti-PARP1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-8000. IP 0.5-4.0 ug for IP and 1:500-1000 for WB. IHC 1:50-500. IF 1:50-500. |
| Target | PARP1 |
| Background | PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1. |
| Swiss Prot | P09874 |
| Gene ID | 142 |
| Subcellular Location | Chromosome Nucleus |
| Immunogen | Recombinant protein of human PARP1 |
| Public Immunogen Range | 666-1014aa |
| Calculated MW | 113kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC IP WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PARP1 antibody diluted at 1:1000;Lane: Jurkat
Immunohistochemistry of paraffin-embedded Human liver cancer with PARP1 antibody diluted at 1:200
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