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My CartCAS：79183-19-0
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：Off-white to yellow Solid
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| CAS | 79183-19-0 |
| Name | Apoptosis Activator 2 |
| Molecular Formula | C15H9Cl2NO2 |
| Molecular Weight | 306.14 |
| Solubility | Soluble in DMSO |
| Purity | ≥98% |
| Appearance | Off-white to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 200-256-5 |
| MDL | MFCD00001743 |
| SMILES | O=C1N(CC2=CC=C(Cl)C(Cl)=C2)C3=C(C=CC=C3)C1=O |
| Target Point | Caspase activator |
| Passage | Apoptosis |
| Background | Apoptosis Activator 2 is a potent activator of apoptosis. |
| Biological Activity | Apoptosis Activator 2强诱导caspase-3激活， PARP分裂， DNA断裂，导致细胞破坏(Apaf-1依赖性的)，IC50约为4 μM，抑制HMEC， PREC和MCF-10A细胞活性。[1-4] |
| In Vitro | Apoptosis Activator 2(20 μM)处于降低的cyto c浓度，增加凋亡体中的Apaf-1组分1.5倍，增至33％。Apoptosis Activator 2(20 μM)处于cyto c的降低水平，增加caspase-3的活化程度4倍。Apoptosis Activator 2强烈诱导caspase-3活化，PARP裂解，和DNA分裂，并最终杀死细胞，IC50为4 μM。Apoptosis Activator 2 诱导PBL，HUVEC，Jurkat，Molt-4，CCRF-CEM，BT-549，MDA-MB-468 和 NCI-H23凋亡，IC50分别为50 μM，43 μM，4 μM，6 μM，9 μM，20 μM，44 μM 和 35 μM。Apoptosis Activator 2抑制大多数测试的肿瘤细胞系，10 μM时抑制50-100%细胞生长。[1]Apoptosis Activator 2通过触发凋亡体的形成而诱导细胞死亡。En1的表达水平对培养的中脑腹侧的生存率没有显著影响。[2]通过凋亡的DNA梯状电泳和TUNEL检测测评Apoptosis activator 2(10 μM)诱导AGS细胞凋亡。Apoptosis activator 2(10 μM)通过anti-TROP2抗体共轭的脂质体，增强对细胞凋亡的诱导。[3] |
| Animal Experiment | Cyclohexamide(10 μg/mL)或 zVAD(50 μM)作用于培养的神经元，显著防止 Apoptosis Activator 2毒性。Apoptosis activator 2(3 μM)导致众多神经元发生核固缩，说明细胞死亡涉及细胞凋亡。DHT(10 nM)或 E2(10 nM)作用于培养的神经元，显著防止Apoptosis Activator 2毒性。[4] |
| Kinase Experiment | 制备HeLa细胞细胞质提取物。Apoptosis Activator 2溶于DMSO中，分加到96孔微量滴定板中，终浓度为1mM(DMSO终浓度为1% vol/vol)。溶于HEB Buffer(50 mM Hepes，pH 7.4/50 mM KCl/5 mM EGTA/2 mM MgCl2)的细胞质提取物的250μg总蛋白，2 mM DTT，2 μM cyto c，和 0.5 μM DEVD-AFC(Asp-Glu-Val-Asp-7-氨基-4-三氟甲基香)底物加到每孔中，总体积为150 μL。[1] |
| Data Literature Source | [1] Nguyen JT，et al. Proc Natl Acad Sci U S A，2003，100(13)，7533-7538. [2] Alavian KN，et al. Neural Dev，2009，16(4)，11. [3] Farivar TN，et al. N Am J Med Sci，2012，4(11)，582-585. [4] Nguyen TV，et al. J Neuroendocrinol，2010，22(9)，1013-1022. |
| Unit | Bottle |
| Specification | 5mg 10mM*1mL in DMSO 10mg 25mg 50mg |
Apoptosis Activator 2是细胞凋亡有效激活剂。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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