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My CartReactivity：Rat,Mouse,Human
Application： WB,ELISA,FCM,IF,ICC,IHC
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Host：Rabbit
Qty：
Size：
| Name | Anti-Histone H3/HIST3H3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Pig;Cow;Rabbit;Fruit Fly |
| Dilution Ratio | WB 1:10000-50000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:100-500. |
| Target | Histone H3/HIST3H3 |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination. |
| Swiss Prot | P68431 |
| Gene ID | 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human Histone H3/HIST3H3 |
| Public Immunogen Range | 71-136/136aa |
| Calculated MW | 15kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with Histone H3 antibody diluted at 1:50000;Lane: Raw264.7 Hela SH-SY5Y Molt-4 THP-1 293T.
Immunohistochemistry of paraffin-embedded Human cervical carcinoma using Histone H3/HIST3H3 antibody diluted at 1:400
Immunofluorescence analysis of Rat brain tissue using H3/HIST3H3 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded human cervical carcinoma with HIST3H3 antibody diluted at 1:400.
Immunofluorescence analysis of human brain using Histone H3 antibody diluted at 1:500.
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