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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-GAPDH Polyclonal AntibodyReactivity：Mouse,Human,Rat
Application： IP,IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-GAPDH Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:10000-30000. IHC 1:50-200. IF 1:50-200. IP 0.5μg-4μg. |
| Target | GAPDH |
| Background | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
| Swiss Prot | P04406 |
| Gene ID | 2597 |
| Subcellular Location | Cytoplasm Membrane Nucleus Cytoskeleton Cytosol Perinuclear region |
| Immunogen | Recombinant protein of human GAPDH |
| Public Immunogen Range | 1-335aa |
| Calculated MW | 31/36kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.03% Proclin300，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GAPDH antibody diluted at 1:5000;Lane: HeLa 293T HepG2.
Immunohistochemistry of paraffin-embedded human colon with GAPDH antibody diluted at 1:75.
Immunofluorescence analysis of HeLa cells using GAPDH antibody diluted at 1:30.
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