{{cart_num}}
My CartCAS：22373-78-0
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to off-white Solid
Qty：
Size：
| CAS | 22373-78-0 |
| Name | Monensin Sodium Salt |
| Molecular Formula | C36H61NaO11 |
| Molecular Weight | 692.85 |
| Solubility | Soluble in Ethanol ≥100mg/mL;Soluble in DMSO ＜ 1mg/mL |
| Purity | ≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 244-941-7 |
| MDL | MFCD26960926 |
| SMILES | OC[C@@]1(O)[C@H](C)C[C@H](C)[C@]([C@@]2(O[C@]([C@@]3(CC[C@@]([C@]4(O[C@@]5(C[C@H](O)[C@@H](C)[C@@]([H])([C@H]([C@@H](OC)[C@H](C)C([O-])=O)C)O5)CC4)C)([H])O3)CC)([H])[C@@H](C)C2)[H])([H])O1.[Na+] |
| InChIKey | XOIQMTLWECTKJL-FBZUZRIGSA-M |
| InChI | InChI=1S/C36H62O11.Na/c1-10-34(31-20(3)16-26(43-31)28-19(2)15-21(4)36(41,18-37)46-28)12-11-27(44-34)33(8)13-14-35(47-33)17-25(38)22(5)30(45-35)23(6)29(42-9)24(7)32(39)40;/h19-31,37-38,41H,10-18H2,1-9H3,(H,39,40);/q;+1/p-1/t19-,20-,21+,22+,23-,24-,25-,26+,27+,28-,29+,30-,31+,33-,34-,35+,36-;/m0./s1 |
| PubChem CID | 23667299 |
| Target Point | Bacterial; Antibiotic; Na+/H+ Exchanger (NHE) |
| Passage | Anti-infection; Membrane Transporter/Ion Channel |
| Background | It is an ionic carrier antibiotic and an ionic carrier that mediates Na+/H+exchange. It is a commonly used protein transport inhibitor, which can destroy Golgi apparatus and inhibit intracellular protein transport. |
| Biological Activity | Monensin sodium salt 是由肉桂链霉菌 (Streptomyces cinnamonensis) 分泌的抗生素。[1-2] |
| In Vitro | 莫能菌素钠盐是由肉桂链霉菌(Streptomyces cinnamonensis)分泌的抗生素。未处理的细胞显示2.5％的细胞凋亡;用1μM莫能菌素钠盐处理48小时显示4.5％的细胞凋亡，而5μM莫能菌素钠盐48小时诱导更大的细胞凋亡反应(16.4％)。与莫能菌素钠盐或厄洛替尼治疗相比，用1或5μM莫能菌素钠盐预处理24小时，然后用10μM厄洛替尼处理另外24小时，导致细胞凋亡事件显著增加(分别为14.6％和38.7％)单独。 5μM莫能菌素钠盐与10μM厄洛替尼的组合显示出最高的细胞凋亡百分比(38.7％)[1]。 |
| In Vivo | 虽然肿瘤数量没有显著变化，但与对照动物相比，莫能菌素钠盐处理的Apc +/Min小鼠的平均肿瘤大小显著减少(P = 0.0144)(平均0.199 mm2对0.299 mm2)。在接受莫能菌素钠盐的个体中，在一只动物中估计的总肿瘤面积减少(平均10.16mm 2对16.46mm 2; P = 0.0125)。莫能菌素钠盐处理增加了在肿瘤生长的表面区域表达p21细胞周期抑制剂的凋亡细胞和细胞的数量。在暴露于莫能菌素钠盐后，注意到粘膜健康部位的细胞增殖，分化和组织结构没有变化[2]。 |
| Cell Experiment | 将100万个SCC25细胞接种在10-cm平板中并孵育过夜以允许附着和恢复。第二天，用0，1或5μM莫能菌素钠盐预处理细胞24小时，然后单独用10μM厄洛替尼或与莫能菌素钠盐组合处理24小时。通过离心收集粘附物和悬浮细胞，并在-20℃下在3mL冷的80％乙醇中固定过夜。在分析之前，将细胞沉淀用PBS洗涤，重悬浮于含有25μg/ mL碘化丙啶和40μg/ mL RNase A的染色缓冲液中，并在室温下在黑暗中孵育至少1小时[1]。 |
| Animal Experiment | 在该研究中使用多个肠道瘤形成(Min)小鼠。四周大的幼仔断奶，基因分型和随机化。将动物分成两组，并用莫能菌素钠盐(10mg/kg)或载体(DMSO)处理。每日口服应用持续6周。此外，用莫能菌素钠盐或载体处理6对7，10，13，16，19和22周龄的Apc +/Min小鼠，持续5周。处死小鼠并解剖肠，用PBS洗涤，并在PBS中的4％甲醛(v/v)中固定3天。固定的肠被包埋在石蜡中，切片并染色。使用Ellipse软件[2]量化肿瘤病变的数量和大小。 |
| Data Literature Source | [1]. Dayekh K，et al. Monensin inhibits epidermal growth factor receptor trafficking and activation: synergistic cytotoxicity in combination with EGFR inhibitors. Mol Cancer Ther. 2014 Nov;13(11):2559-71. [2]. Tumova L，et al. Monensin inhibits canonical Wnt signaling in human colorectal cancer cells and suppresses tumor growth in multiple intestinal neoplasia mice. Mol Cancer Ther. 2014 Apr;13(4):812-22. |
| Unit | Piece |
| Specification | 100mg 200mg |
Monensin sodium salt is an ionic carrier antibiotic, which is a mediating agent. Na+/H+ The ionic carrier of exchange. Monensin is a commonly used protein transport inhibitor that destroys the Golgi apparatus, inhibits intracellular protein transport, and is often used to inhibit the secretion of secreted proteins such as cytokines, so that they remain in the cell.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
Manual Download
