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My CartApplication：ELISA
Reactivity：Common Species
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：0.35 ng/ml
Storage：2-8℃，6 months
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| Name | HA ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Hyaluronan |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Common Species |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 0.7-45 ng/ml |
| Sensitivity | 0.35 ng/ml |
| Specificity | Do not react with bilirubin, hemoglobin, heparin etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Detection Principle | Solarbio ELISA试剂盒采用基于夹心法的酶联免疫吸附检测技术。将重组人可聚蛋白多糖(recombinant human Aggrecan)包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的Hyaluronan会与酶标板上包被的重组人可聚蛋白多糖充分结合；洗板后加入生物素化的重组人可聚蛋白多糖(biotinylated recombinant human Aggrecan)，该可聚蛋白多糖会与板子上包被的重组人可聚蛋白多糖捕获的标准品和样本中的Hyaluronan发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的Hyaluronan，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的Hyaluronan浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中Hyaluronan的浓度。 |
| Background | Hyaluronan，also known as hyaluronic acid (HA) or sodium hyaluronate，is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1，2，and 3)，which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic，anti-inflammatory and immunosuppressive，low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally，hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues，which is involved in cell adhesion and supports cell migration. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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