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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：47 pg/ml
Storage：2-8℃，6 months
Gene ID：26525
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| Name | Human IL-36RA ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-36RA |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 93.75-6000 pg/ml |
| Sensitivity | 47 pg/ml |
| Specificity | Do not react with Human IL-1β， Human IL-1F7， Human IL-1ra， Human IL-1RAcP/FcChimera， Human IL-1Rrp2/FcChimera， Human IL-36α， Human IL-36β， Human IL-36γ， Mouse IL-36Ra etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q9UBH0 |
| Gene ID | 26525 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人 IL-36RA/IL-1F5单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人 IL-36RA/IL-1F5会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人 IL-36RA/IL-1F5抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人 IL-36RA/IL-1F5发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人 IL-36RA/IL-1F5，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人 IL-36RA/IL-1F5浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人 IL-36RA/IL-1F5的浓度。 |
| Background | Interleukin 36 Receptor Antagonist (IL-36Ra)，also known as IL-1F5，FIL1 delta，IL-1HY1，IL-1H3，and IL-1L1，is a member of the IL-1 family of proteins. All family members show a 12 beta-strand，beta-trefoil configuration，and all are believed to have arisen from a common ancestral gene that underwent multiple duplications. The human IL-36Ra gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of IL-1ra. Cells reported to express IL-36Ra include monocytes，B cells，dendritic cells/Langerhans cells，keratinocytes，and gastric fundus parietal and chief cells. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
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