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My CartApplication：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：21 pg/ml
Storage：2-8℃，6 months
Gene ID：5327
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| Name | Human tPA ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | tPA |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 78.12-5000 pg/ml |
| Sensitivity | 21 pg/ml |
| Specificity | Do not react with Human CoagulationFactorX， Human Fibronectin， Human LRP-1， Human LRP-1B， Human SerpinA1， Human SerpinA3， Human SerpinA4， Human SerpinA5， Human SerpinA6 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | P00750 |
| Gene ID | 5327 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人tPA单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人tPA会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人tPA抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人tPA发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人tPA，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人tPA浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人tPA的浓度。 |
| Background | Tissue Plasminogen Activator (tPA)，also known as PLAT，is a 64-69 kDa extracellular glycoprotein that belongs to the peptidase S1 family of serine proteases. The biological effects of tPA include blood clot degradation，vascular remodeling，synaptic plasticity，and neurodegeneration in the brain following trauma. Human tPA is secreted as a 530 amino acid (aa) single chain polypeptide (36-562 aa) that contains an amino-terminal fibrin-finger-like domain，an epidermal growth factor-like domain，two kringle domains and a C-terminal serine protease catalytic domain (1，2). The partially active single chain tPA is cleaved between Arg310-Ile311 by Plasmin，Kallikrein/KLKB1，and Coagulation Factor X/Xa to generate the mature two-chain disulfide-linked polypeptide. This mature form of tPA is 10-fold more catalytically active than the single chain (3，4). From aa 36-562，human tPA shares 81% and 72% identity with mouse and rat tPA，respectively. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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