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My CartCAS：5289-74-7
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to yellow Solid
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| CAS | 5289-74-7 |
| Name | Ecdysterone |
| Molecular Formula | C27H44O7 |
| Molecular Weight | 480.63 |
| Solubility | Soluble in DMSO≥100mg/mL；Soluble in Ethanol≥5mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 811-097-1 |
| MDL | MFCD00036740 |
| SMILES | O[C@@H]1[C@H](O)C[C@](C(C=C2[C@]3([H])CC[C@@]4(C)[C@@]2(O)CC[C@]4([H])[C@](O)(C)[C@H](O)CCC(O)(C)C)=O)([H])[C@]3(C)C1 |
| Target Point | Caspase; Autophagy |
| Passage | Apoptosis; Autophagy |
| Background | Ecdysone is an ecdysone that controls molting and metamorphosis in arthropods, inhibits caspase activity and induces autophagy through the 20E nuclear receptor complex EcR-USP. |
| Biological Activity | 20-Hydroxyecdysone (Ecdysterone, 20E, B-ecdysone, Commisterone, Crustecdysone) 是一种自然发生的蜕皮激素，控制节肢动物的蜕皮和变态。20-Hydroxyecdysone (Ecdysterone, 20E) 可抑制 caspase 活性并通过20E核受体复合体 EcR-USP 来诱导自噬。[1-5] |
| In Vitro | 20-Hydroxyecdysone处理 H4IIE细胞培养物，降低磷酸烯醇式丙酮酸羧化酶(PEPCK)和 葡萄糖-6-磷酸(G6Pase)的表达，降低葡萄糖产量，且诱导Akt2磷酸化对 PI3K通路特定抑制剂LY-294002的敏感性。[1] 在CoCl2处理前后使用20-Hydroxyecdysone 处理，显著改善 CoCl2诱导的细胞损伤。20-Hydroxyecdysone显著降低CoCl2-诱导的活性氧(ROS)产量，降低线粒体膜的去极化，抑制 细胞色素 c释放到细胞质中，提高Bax/Bcl-2比率，且消除CoCl2诱导的 caspase-3激活。 [2] 在体外，使用20-Hydroxyecdysone处理幼虫脂肪体组织，上调凋亡基因，包括Apaf-1，Nedd2 like1，Nedd2 like2，ICE1，ICE3，ICE5，Arp，和 IAP。[4]20-Hydroxyecdysone 逆转TGF-β1诱导的细胞内外纤连蛋白增多，也逆转 TGF-β1诱导的Smad7下调。此外，20-Hydroxyecdysone显著降低TGF-β1诱导的Smad2/3 和 pSmad2/3上调，以及E-Cadherin的下调。而且，20-Hydroxyecdysone显著抑制TGF-β1诱导的 Snail表达提高。[5] |
| In Vivo | 20-Hydroxyecdysone注射到蚕，中国桑蚕的脂肪体中，处理6小时后，显著诱导凋亡和上调凋亡基因。[4] |
| Cell Experiment | 20-Hydroxyecdysone 每天按10 mg/kg剂量口服处理高脂肪饮食饲喂的C57BL/6J小鼠，持续13天，改善肥胖和胰岛素抗性，与未处理的对照组相比，体重显著减轻，且身体脂肪量显著降低。此外，20-Hydroxyecdysone 处理后，血浆胰岛素水平和耐糖量显著降低。这种改变伴随着肝脏PEPCK和G6Pase表达降低，和内脏脂肪组织产生的脂联素增高。[1] |
| Animal Experiment | 20-Hydroxyecdysone 作用于稳定转染IL-6结合报告基因的HeLa 细胞，抑制NF-κB活化，IC50为31.8 μM。[3] |
| Data Literature Source | [1] Kizelsztein P，et al. Am J Physiol Endocrinol Metab，2009，296(3)，E433-439. [2] Hu J，et al. J Cell Biochem，2010，111(6)，1512-1521. [3] Peschel W，et al. J Pharm Pharmacol，2011，63(11)，1483-1495. [4] Tian L，et al. Arch Insect Biochem Physiol，2012，79(4-5)，207-219. [5] Hung TJ，et al. J Diabetes Complications，2012. |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg 100mg |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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