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My CartCAS：2627-69-2
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to brown Solid
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| CAS | 2627-69-2 |
| Name | Acadesine |
| Molecular Formula | C9H14N4O5 |
| Molecular Weight | 258.23 |
| Solubility | Soluble in Water/DMSO |
| Purity | ≥98% |
| Appearance | White to brown Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| Delivery Time | 1-2 Days |
| EC | EINECS 220-097-5 |
| MDL | MFCD00869751 |
| SMILES | OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC(C(N)=O)=C2N)O1 |
| InChIKey | RTRQQBHATOEIAF-UUOKFMHZSA-N |
| InChI | InChI=1S/C9H14N4O5/c10-7-4(8(11)17)12-2-13(7)9-6(16)5(15)3(1-14)18-9/h2-3,5-6,9,14-16H,1,10H2,(H2,11,17)/t3-,5-,6-,9-/m1/s1 |
| PubChem CID | 17513 |
| Target Point | AMPK;Autophagy;YAP;Mitophagy |
| Passage | PI3K/Akt/mTOR;Epigenetics |
| Background | Acadisine is an adenosine analog and an AMPK activator. Acadisine is also an autophagy, YAP and mitophagy inhibitor. |
| Biological Activity | AICAR是一种可渗透细胞的AMP活化蛋白激酶 (AMPK) 激活剂。[1-4] |
| In Vitro | 用不同浓度的AICAR(0.1-1.0mM)处理HepG2细胞12，24和48小时。 IR-β的表达水平在对照组的48小时至50％，53％和46％时分别以0.25，0.5和1.0mM的AICAR显着降低[1]。 |
| In Vivo | 将14周龄雄性，瘦肉(L; 31.3g体重)野生型andob / ob(O; 59.6g体重)小鼠注射AMP激活激酶(AMPK)激活剂AICAR(A)0.5mg / g /天或盐水对照(C)14天。在最后一次注射后24小时(包括12小时禁食)，杀死所有小鼠，切除足底屈肌复合肌(腓肠肌，比目鱼肌和跖肌)进行分析。 OC(159±12 mg)的肌肉质量低于LC，LA和OA(分别为176±10，178±9和166±16 mg)小鼠，与体重变化无关[2]。与运动和AICAR(0.5mg / g体重)组相比，未治疗组的肾重量显着更高。运动组的心脏重量高于其他组，而AICAR治疗组的肝脏重量显着高于运动组和未治疗组[3]。 |
| Cell Experiment | 将HepG2细胞(5×10 5个细胞)接种在6孔培养板培养皿中，然后在无血清培养基中培养12小时，然后转染。用FuGENE6转染试剂转染1微克质粒。转染5小时后，除去培养基，然后向每个孔中加入补充有或不含AICAR(0.1-1.0mM)的培养基。刺激介质每24小时更换一次[1]。 |
| Animal Experiment | 小鼠[2]使用14周龄瘦(Lepob / +或Lepob / +)和ob / ob(Lepob / Lepob)雄性小鼠。经过14天的实验治疗(AICAR注射后24小时，包括12小时禁食)，足底屈肌复合肌从4％异氟醚麻醉的小鼠切除干净(肌腱 - 肌腱)。快速称重肌肉，然后进行组织学处理或在液氮中冷冻并储存在-80℃。在通过针刺直接进入心脏采血后，通过隔膜横切和整个心脏的移除来杀死麻醉的小鼠，同时呼吸4％的异氟烷。将AICAR或盐水(对照)皮下注射到背部的侧向远侧部分。 AICAR以0.5mg / g每天一次给药，持续14天。盐水(对照)以与AICAR治疗相同的方式注射，其用量与AICAR治疗相同。在死亡之前测量体重。大鼠[3]雄性5周龄ZDF大鼠皮下注射单剂量AICAR(0.5 mg / g体重)或经历一次跑步机运行(60分钟，速度25 m / min)5％倾斜)。未处理的ZDF大鼠用作对照(每组n = 5)。皮下AICAR注射后1小时或在跑步机运行后立即，通过颈椎脱位将大鼠杀死。为了避免肌肉痉挛和缺氧的任何影响，在几秒钟内移除红色和白色腓肠肌并立即冷冻夹紧以便随后确定AMPK活性。 |
| Data Literature Source | [1]. Nakamaru K， et al. AICAR， an activator of AMP-activated protein kinase， down-regulates the insulin receptor expression in HepG2 cells. Biochem Biophys Res Commun. 2005 Mar 11; 328 (2) :449-54 [2]. Drake JC， et al. AICAR treatment for 14 days normalizes obesity-induced dysregulation of TORC1 signaling and translational capacity in fasted skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2010 Dec; 299 (6) :R1546-54. [3]. Pold R， et al. Long-term AICAR administration and exercise prevents diabetes in ZDF rats.Diabetes. 2005 Apr; 54 (4) :928-34. [4]. Chen L， et al. Activating AMPK to Restore Tight Junction Assembly in Intestinal Epithelium and to Attenuate Experimental Colitis by Metformin. Front Pharmacol. 2018 Jul 16; 9:761. |
| Unit | Bottle |
| Specification | 10mM*1mL in DMSO 50mg 100mg 500mg |
阿卡地新是一种腺苷类似物，也是一种 AMPK 激活剂。阿卡地新也是一种自噬 (autophagy)，YAP 和mitophagy抑制剂。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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