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My CartApplication：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：78.13 pg/ml
Storage：2-8℃，6 months
Gene ID：216799
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| Name | Mouse NLRP3/NALP3/CIAS1/PYPAF ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | NLRP3/NALP3/CIAS1/PYPAF |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 156.25-10000 pg/ml |
| Sensitivity | 78.13 pg/ml |
| Specificity | Do not react with Mouse BMP1，BMP2，BMP3，BMP4，IL-1β，IL-4，IL-5，IL-6，IL-8，IFN-γ，TGF-β1，TGF-β2，TGF-β3，TLR1，TLR2，TLR3， Human 的NLRP3 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | Q8R4B8 |
| Gene ID | 216799 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠NLRP3单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠NLRP3会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠NLRP3抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠NLRP3发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠NLRP3，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠NLRP3浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠NLRP3的浓度。 |
| Background | NLRP3，also known as NALP3，CIAS1，PYPAF or Cryopyrin，is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes，especially neutrophils. As a component of the inflammasome，NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1，CINCA，or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532)，and seven LRRs (aa 739-988). Within the sequence used as an immunogen，mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3，respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3，LRR 6 and 7，or LRR4-9. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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