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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：125-8000 pg/ml
Storage：2-8℃，6 months
Gene ID：2255
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| Name | Human FGF10 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | FGF10 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 125-8000 pg/ml |
| Sensitivity | 125-8000 pg/ml |
| Specificity | Do not react with Human ApoAI，BMP1，BMP2，BMP4，BMP7，HGF，HSP27，IL-1β，IL-1RA，IL-2，IL-2R，IL-5，IL-6，IL-6R，IL-8，IL-10，IL-12，IL-13，IL-15，IL-17C，IL-23，IFNγ，MMP-2，MMP-9，PDGF-AA，PDGF-BB，PDGF-AB，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR3，TNF-α，TNFRI，TNFRII etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | O15520 |
| Gene ID | 2255 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人FGF10单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人FGF10会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人FGF10抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人FGF10发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人FGF10，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人FGF10浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人FGF10的浓度。 |
| Background | The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal，neuronal，and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3，-7，and -22. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10，respectively. FGF-10 is secreted by mesenchymal cells and associates with?extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF?R2 (IIIb) and interacts more weakly with FGF?R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation，palate development，branching morphogenesis and directional outgrowth of lung buds，formation of the otic vesicle and chochlea，adipogenesis，and the development of prostate，mammary，lacrimal，and submandibular salivary glands. FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3，-7，?and -8 suggest at least a partial redundancy in FGF?10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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Delivery of FGF10 by implantable porous gelatin microspheres for treatment of spinal cord injury
Click to check >>Author：Yuntao Gu, Guangyu Wen, Hai Zhao, Hao Qi, Yi Yang, Tianqiong Hu
IF：3.4230
Publish_to：Molecular Medicine Reports
PMID：37264963
