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My CartCAS：3778-73-2
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥99%
Appearance：White to off-white Solid
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| CAS | 3778-73-2 |
| Name | Ifosfamide |
| Molecular Formula | C7H15Cl2N2O2P |
| Molecular Weight | 261.09 |
| Solubility | Soluble in DMSO ≥5mg/mL(Need ultrasonic) |
| Purity | ≥99% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 223-237-3 |
| MDL | MFCD00057374 |
| SMILES | ClCCN1P(OCCC1)(NCCCl)=O |
| InChIKey | HOMGKSMUEGBAAB-UHFFFAOYSA-N |
| InChI | InChI=1S/C7H15Cl2N2O2P/c8-2-4-10-14(12)11(6-3-9)5-1-7-13-14/h1-7H2,(H,10,12) |
| PubChem CID | 3690 |
| Target Point | DNA Alkylator |
| Passage | DNA Damage/DNA Repair |
| Background | Idramantone is an immune stimulant that can be used in research related to neurological diseases. |
| Biological Activity | Ifosfamide 是一种烷化剂 (DNA alkylator)，具有广谱的抗肿瘤作用。[1-3] |
| In Vitro | 异环磷酰胺是一种烷化化学治疗剂，具有抗多种肿瘤的作用[1]。异环磷酰胺被细胞色素P450家族激活。异环磷酰胺(0-5 mM)抑制表达CYP2B1的C8III-1细胞的活力，而仅在高浓度(5 mM)时对表达非CYP2B1的CrFK细胞具有细胞毒性[2]。 CYP BM3突变体激活异环磷酰胺，异环磷酰胺对人U2OS细胞具有抑制活性[3]。 |
| In Vivo | 交配前的异环磷酰胺(50mg / kg，ip)处理增加了植入后损失的百分比并且在怀孕大鼠中吸收胎儿。异环磷酰胺(50mg / kg，ip)也降低了怀孕大鼠血清中的孕酮。然而，异环磷酰胺与25mg / kg对照大鼠没有明显差异。异环磷酰胺(25，50 mg / kg，ip)诱导胎盘和产前大鼠的细胞凋亡和组织学变化，最敏感的胎儿器官是脑，肝和肾[1]。 |
| Cell Experiment | 在3cm培养皿中，将4×104个细胞接种在2mL培养基中。第二天，加入异环磷酰胺至终浓度为0至5 mM。再过6天后，除去培养基，用PBS洗涤细胞，染色或计数[2]。 |
| Animal Experiment | 大鼠[1]在交配之前，将雌性大鼠分成四组，每组八只：第1组，未治疗的阴性对照; 第2组，大鼠腹腔注射1 mL 0.9％NaCl; 第3组，大鼠腹腔注射25 mg / kg异环磷酰胺; 第4组，大鼠腹腔注射50 mg / kg异环磷酰胺。在每天一次注射异环磷酰胺连续5天后，将三只雌性与一只未经治疗的雄性一起放置在笼子中长达1周。每天检查阴道涂片以确定怀孕。如果检测到精子，则在交配后的第一个24小时期间被指定为怀孕第一天。怀孕的雌性单独饲养，每天观察有毒性和流产的迹象。在妊娠第18天通过断头处死所有怀孕动物。在非肝素化试管中收集心脏血液(2.5-3mL /大鼠)，以3，000×g离心30分钟，倾析血清并储存在-70℃直至用于激素测定。收集血液后，取出子宫和两个卵巢，用盐水溶液洗涤，目测计数妊娠黄体，并计算每个子宫角的植入部位，再吸收部位和活胎的数量。将每个胎儿从其脐带中取出，称重并测量冠臀(CR)长度。检查胎儿的外部畸形并记录胎盘重量。对照组和治疗组的胎儿和胎盘用10％中性缓冲福尔马林固定，用于免疫组化和组织学检查[1]。 |
| Data Literature Source | [1]. Helal M. Prenatal effects of transplacental exposure to ifosfamide in rats. Biotech Histochem. 2016 Jul; 91 (5) :357-68. [2]. Karle P， et al. Necrotic， rather than apoptotic， cell death caused by cytochrome P450-activated ifosfamide. Cancer Gene Ther. 2001 Mar; 8 (3) :220-30. [3]. Vredenburg G， et al. Activation of the anticancer drugs cyclophosphamide and ifosfamide by cytochrome P450 BM3 mutants. Toxicol Lett. 2015 Jan 5; 232 (1) :182-92 |
| Unit | Bottle |
| Specification | 100mg 200mg 500mg |
Ifosfamide 是一种烷化剂 (DNA alkylator)，具有广谱的抗肿瘤活性。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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