β-Carotene Content HPLC Assay kit
Cat.No：BC4644 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Other Series > β-Carotene Content HPLC Assay kit
Product Information
| Name | β-Carotene Content HPLC Assay kit |
| Detection Method | HPLC |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
β-carotene, one of the carotenoids, is an orange-yellow fat-soluble compound that is ubiquitous in nature and is the most stable natural pigment. Many natural foods such as green vegetables, sweet potatoes, carrots, spinach, papaya, mangoes, etc., are rich in β-carotene.
Measurement Principle：
PEPCK catalyzes oxaloacetic acid to form phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+ in turn, and determine the NADH decline rate at 340 nm, which can reflect the PEPCK activity.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, water bath pot, micro quartz cuvette/96 well flat-bottom plate (UV), adjustable pipette, mortar/homogenizer, ice and distilled water
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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