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My CartCAS：126-19-2
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 126-19-2 |
| Name | Sarsasapogenin |
| Molecular Formula | C27H44O3 |
| Molecular Weight | 416.64 |
| Solubility | Soluble in Ethanol;Soluble in DMSO(Need ultrasonic or Water bath) |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 204-776-3 |
| MDL | MFCD00270414 |
| SMILES | C[C@@H]1[C@]2(OC[C@@H](C)CC2)O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC[C@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]31[H] |
| Target Point | NF-kB |
| Passage | NF-κB |
| Background | Sarsasapogenin is a steroid saponin with anti-diabetes， anti-oxidation， anti-cancer and anti-inflammatory activities. |
| Biological Activity | Sarsasapogenin 是从中草药知母中分离到的甾体皂苷元，具有抗糖尿病、抗氧化、抗癌、抗炎等多种功效。[1-3] |
| In Vitro | 萨洒皂草配基(20-80μM)通过heLa细胞中的半胱天冬酶依赖性线粒体途径诱导细胞凋亡。萨洒皂草配基还通过半胱天冬酶依赖性线粒体凋亡途径诱导细胞凋亡。萨洒皂草配基(60μM)诱导的ROS产生引发ER应激和线粒体功能障碍[1]。 Sarsasapogenin有效抑制LPS刺激的巨噬细胞中的NF-κB和MAPK活化，以及IRAK1，TAK1和IκBα磷酸化。此外，Sarsasapogenin抑制LPS与巨噬细胞Toll样受体4的结合，以及M2与M1巨噬细胞的极化[3]。 |
| In Vivo | 萨洒皂草配基(20和40mg/kg)显著恢复由嗅球切除术(OB)诱导的蔗糖偏好缺陷，并增加运动活性。萨洒皂草配基组(20和40 mg/kg)具有显著更低的固定时间，更高的AChE蛋白表达水平比OB组。此外，与OB组大鼠相比，Sarsasapogenin(20和40 mg/kg)组具有显著更高的α7-nAChR蛋白表达，并且增加更高的α4-nAChR蛋白表达水平[2]。 Sarsasapogenin(5或10 mg/kg，po)抑制TNBS诱导的小鼠结肠缩短和髓过氧化物酶活性，减少NF-κB活化和白细胞介素(IL)-1β，肿瘤坏死因子(TNF)-α和IL-6水平，同时增加IL-10 [3]。 |
| Cell Experiment | 通过MTT测定法测量萨洒皂草配基对细胞活力的影响。在不存在或存在Z-VAD-FMK预处理指定时间的情况下暴露于萨洒皂草配基后，将96孔板中的细胞与MTT一起温育。将甲crystals沉淀物溶解在200μL二甲基亚砜中，并使用Benchmark酶标仪测量570nm处的吸光度。 |
| Animal Experiment | 将小鼠随机分为7组：正常对照组，用载体治疗的TNBS诱导的结肠对照组，知母皂苷AIII(5或10 mg/kg)，萨洒皂草配基(5或10 mg/kg)或柳氮磺胺吡啶(50 mg/kg)。每组由6只小鼠组成。通过使用配备有注射器的薄圆头针直肠内注射2.5％(w/v)TNBS溶液(100μL，溶于50％乙醇)到麻醉小鼠的结肠中来诱导结肠炎。正常组仅用载体而不是TNBS和测试剂治疗。如果注射的TNBS溶液被排出，则将小鼠排除在实验之外。在用TNBS处理后，溶解在2％吐温80中的Timosaponin AIII或萨洒皂草配基(5或10mg/kg)或柳氮磺吡啶(50mg/kg)每天一次口服给药，持续3天。在最后施用测试剂后18小时杀死小鼠。快速取出结肠，纵向打开，并用PBS轻轻洗涤。评估结肠炎等级的宏观评价(0，无溃疡和无炎症; 1，无溃疡和局部充血; 2，溃疡充血; 3，仅在一个部位溃疡和炎症; 4，两个或更多溃疡部位和炎症; 5，溃疡延伸超过2厘米)。将结肠储存在-80℃直至用于ELISA和免疫印迹的实验中。 |
| Data Literature Source | [1]. Shen S，et al. Sarsasapogenin induces apoptosis via the reactive oxygen species-mediated mitochondrial pathway and ER stress pathway in HeLa cells. Biochem Biophys Res Commun. 2013 Oct 26. [2]. Feng B，et al. Sarsasapogenin reverses depressive-like behaviors and nicotinic acetylcholine receptors induced by olfactory bulbectomy. Neurosci Lett. 2017 Feb 3;639:173-178. [3]. Lim SM，et al. Timosaponin AIII and its metabolite sarsasapogenin ameliorate colitis in mice by inhibiting NF-κB and MAPK activation and restoring Th17/Treg cell balance. Int Immunopharmacol. 2015 Apr;25(2):493-503 |
| Unit | Bottle |
| Specification | 5mg 10mg 20mg |
具有抗糖尿病、抗氧化、抗癌、抗炎等多种活性。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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