Rat MPO ELISA Kit
Cat.No：SEKR-0073 Solarbio
Application： ELISA
Reactivity：Rat
Sensitivity：1.6 ng/ml
Storage：2-8℃，6 months
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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Product Information
| Name | Rat MPO ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Myeloperoxidase/MPO |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 3.125-200 ng/ml |
| Sensitivity | 1.6 ng/ml |
| Specificity | Do not react with Rat ApoAI， Rat BMP1， Rat BMP2， Rat BMP4， Rat BMP7， Rat HGF， Rat HSP27， Rat IL-1β， Rat IL-1RA， Rat IL-2， Rat IL-2R， Rat IL-5， Rat IL-6， Rat MMP-2， Rat MMP-9， Rat PDGF-AA，etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | D3ZGE2 |
| Gene ID | 303413 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠Myeloperoxidase/MPO单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠Myeloperoxidase/MPO会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠Myeloperoxidase/MPO抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠Myeloperoxidase/MPO发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠Myeloperoxidase/MPO，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠Myeloperoxidase/MPO浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠Myeloperoxidase/MPO的浓度。 |
| Background | Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin，MMR，Cytokeratin 1 on vascular endothelial cells，HMW Kininogen，and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance，a reduction of nitric oxide and bradykinin levels，reduced vasodilation，and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation，MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation，eclampsia，risk of cardiovascular events，vascular endothelial dysfunction，severity of multiple sclerosis，and prospective mortality and oxidative stress during hemodialysis. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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