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My CartApplication：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：31.25 pg/ml
Storage：2-8℃，6 months
Gene ID：11576
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| Name | Mouse AFP ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | AFP |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 62.5-4000 pg/ml |
| Sensitivity | 31.25 pg/ml |
| Specificity | Do not react with Mouse Leptin，Prolactin，Resistin，VitaminDBP， Human AFP etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P02772 |
| Gene ID | 11576 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠AFP单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠AFP会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠AFP抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠AFP发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠AFP，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠AFP浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠AFP的浓度。 |
| Background | alpha-Fetoprotein (AFP)，is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver，yolk sac，and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids，bilirubin，fatty acids，retinoids，and flavonoids. In addition，it can exert immunosuppressive activity，regulate cell proliferation and apoptosis，initiate intracellular signaling，and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism，autoimmune disorders，and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome，whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver，stomach，and germ cell cancers. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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