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My CartCAS：121-34-6
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to yellow Solid
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| CAS | 121-34-6 |
| Name | Vanillic acid |
| Molecular Formula | C8H8O4 |
| Molecular Weight | 168.15 |
| Solubility | Soluble in Ethanol/DMSO ≥20mg/mL；Soluble in Water＜2mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 204-466-8 |
| MDL | MFCD00002551 |
| SMILES | O=C(O)C1=CC=C(O)C(OC)=C1 |
| InChIKey | WKOLLVMJNQIZCI-UHFFFAOYSA-N |
| InChI | InChI=1S/C8H8O4/c1-12-7-4-5(8(10)11)2-3-6(7)9/h2-4,9H,1H3,(H,10,11) |
| PubChem CID | 8468 |
| Target Point | NF-KB |
| Passage | NF-κB |
| Background | Vanillic acid inhibits NF-κB activation. Has anti-inflammatory, antibacterial and other activities. |
| Biological Activity | Vanillic acid 是一种在食用植物和水果中发现的调味剂。Vanillic acid 抑制 NF-κB 活化。具有抗炎，抗菌等活性。[1-2] |
| In Vitro | 通过Western印迹测定，香草酸抑制脂多糖(LPS)诱导的细胞质提取物中IκB(细胞质NF-κB抑制剂)的磷酸化和体外巨噬细胞核提取物中NF-κBp65亚基的表达[1]。香草酸处理可逆转Aβ1-42诱导的IKK-β/ NF-κB表达升高，并降低IKKβ/ NF-κB的活性。在所有三种浓度(50，100和200μM)下，香草酸对HT22细胞无毒;与Aβ1-42的香草酸共处理显著增加(分别为1.5倍，1.9倍和2倍)细胞活力。与对照细胞相比，Aβ1-42(5μM)处理导致ROS水平显著增加(1.6倍)。相比之下，所有三种不同浓度(50，100和200μM)的香草酸处理均降低(分别为1.1倍，1.3倍和1.4倍)ROS水平，表明香草酸是一种有效的抗氧化剂[2]。 |
| In Vivo | 香草酸(30mg/kg，ip，1h)抑制角叉菜胶诱导的NF-κB活化。小鼠在ip注射角叉菜胶(300μg/爪)前1小时接受香草酸(30mg/kg，ip)，并在刺激注射后3小时收集皮肤足底组织的样品。卡拉胶诱导的NF-κB活化通过总NF-κB/ p-NF-κB比例降低来观察，而用香草酸和对照药物吲哚美辛(5 mg/kg，ip，稀释)进行治疗。刺激前40分钟的Tris/HCl缓冲液抑制角叉菜胶诱导的NF-κB活化[1]。 |
| Cell Experiment | 用MTT测定评估HT22细胞的活力。将细胞在96孔板中以每孔1×10 4个细胞的密度在100μLDulbecco改良的Eagle培养基(DMEM)中培养。 24小时后，用含有Aβ1-42(5μM)的新鲜培养基替换培养基，其中三种不同浓度(50，100和200μM)的香草酸单独或与Aβ1-42(5μM)组合，并且将细胞再孵育24小时。对照细胞仅接收DMEM。此后，将细胞与MTT溶液在37℃下再孵育2-4小时。随后，用DMSO替换每个孔中的培养基。最后，使用ApoTox仪器测量570nm处每个孔中溶液的吸光度。所有实验均一式三份进行[2]。 |
| Animal Experiment | 小鼠[1]在实验过程中，雄性瑞士小鼠(25-30g)在给予香草酸或载体(5％吐温80稀释于盐水中)前1小时或24小时接受腹膜内(3，10或30mg/kg)治疗。 h炎症刺激注射后[1]。 |
| Data Literature Source | 1]. Calixto-Campos C，et al. Vanillic acid Inhibits Inflammatory Pain by Inhibiting Neutrophil Recruitment，Oxidative Stress，Cytokine Production，and NFκB Activation in Mice. J Nat Prod. 2015 Aug 28;78(8):1799-808. [2]. Amin FU，et al. Vanillic acid attenuates Aβ1-42-induced oxidative stress and cognitive impairment in mice. Sci Rep. 2017 Jan 18;7:40753 |
| Unit | Piece |
| Specification | 100mg 10mM*1mL in DMSO 500mg |
Vanillic acid 可以抑制 NF-κB 活化。具有抗炎，抗菌等活性。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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