{{cart_num}}
My Cart> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Immunology & Inflammation > RutaecarpineCAS：84-26-4
Molecular Formula：C18H13N3O
Molecular Weight：287.32
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
Qty：
Size：
| CAS | 84-26-4 |
| Name | Rutaecarpine |
| Molecular Formula | C18H13N3O |
| Molecular Weight | 287.32 |
| Solubility | Soluble in DMSO |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 635-907-6 |
| MDL | MFCD00210551 |
| SMILES | O=C1N2C(C(NC3=C4C=CC=C3)=C4CC2)=NC5=C1C=CC=C5 |
| Target Point | COX |
| Passage | Immunology & Inflammation |
| Background | Rutaecarpine is a COX-2 inhibitor. |
| Biological Activity | Rutaecarpine, 是槐木的生物碱，是COX-2的抑制剂，其IC50值为0.28 μM.[1-3] |
| IC50 | COX-2:0.28μM( BMMC);COX-1:8.7μM(BMMC)[1-3] |
| In Vitro | Rutaecarpine已显示出多种有趣的生物学特性，如抗血栓形成，抗癌，抗炎和镇痛，抗肥胖和体温调节，血管舒张活性，以及对心血管和内分泌系统的影响[2]。 Rutaecarpine以浓度依赖性方式抑制BMMC中PGD2产生的COX-2和COX-1依赖性相，IC50分别为0.28μM和8.7μM。它通过COX-2转染的HEK293细胞以剂量依赖性方式抑制外源性花生四烯酸向PGE2的COX-2依赖性转化[1]。 |
| In Vivo | Rutaecarpine通过腹膜内给药显示出对大鼠L-角叉菜胶诱导的爪水肿的体内抗炎活性[1]。 Rutaecarpine显著降低抗体形成细胞的数量，并以剂量依赖性方式引起脾脏重量减少。此外，施用吴茱萸次碱的小鼠脾脏细胞数减少，总T细胞数量减少，CD4 +细胞数量增加，CD8 +细胞减少，脾脏中出现B细胞减少。通过吴茱萸次碱处理显著抑制IL-2，干扰素和IL-10mRNA表达。给予rutaecarpine小鼠后，CD4 + IL-2 +细胞数量显著减少[3]。 |
| Cell Experiment | 将Rutaecarpine溶解于DMSO中，并在使用前用适当的培养基稀释。制备COX-1和COX-2 cDNA转染的HEK293细胞。为了测量由吴茱萸次碱对COX-1和COX-2的抑制活性，将1mL培养基中的细胞接种到24孔的每个孔中。培养4天后，除去上清液，向有或没有吴茱萸次碱的细胞中加入250mL新鲜培养基。在37℃预温育5小时后，将细胞在50℃下用50mM花生四烯酸进一步在37℃温育30分钟。通过在4℃下以120g离心5分钟来终止所有反应。测量上清液中PGE2的浓度[1]。 |
| Animal Experiment | 大鼠：将Rutaecarpine溶解在0.1％羧甲基纤维素中，并在使用前用适当的培养基稀释。在该研究中使用雄性Splague-Dawley(SD)大鼠(180-220g)。腹膜内施用吴茱萸次碱，1小时后，将1-角叉菜胶溶液注射到大鼠的右后爪中。在1-角叉菜胶注射后5小时使用体积描记器测量爪体积[1]。小鼠：对于对SRBC的抗体反应，静脉内以10mL 1％聚维酮溶液中的10mg/kg，20mg/kg，40mg/kg或80mg/kg的单剂量施用吴茱萸次碱。对照动物以10mL/kg给予1％聚维酮溶液。在研究中使用无特定病原体的雌性BALB/c小鼠[3]。 |
| Data Literature Source | [1]. Moon TC，et al. A new class of COX-2 inhibitor，rutaecarpine from Evodia rutaecarpa. Inflamm Res. 1999 Dec;48(12):621-5. [2]. Lee SH，et al. Progress in the studies on rutaecarpine. Molecules. 2008 Feb 6;13(2):272-300. [3]. Jeon TW，et al. Immunosuppressive effects of rutaecarpine in female BALB/c mice.Toxicol Lett. 2006 Jul 1;164(2):155-66. |
| Unit | Bottle |
| Specification | 20mg 10mM*1mL in DMSO 100mg |
Rutaecarpine是COX-2的抑制剂.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
