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My CartCAS：81525-13-5
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to yellow Solid
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| CAS | 81525-13-5 |
| Name | Forsythoside B |
| Molecular Formula | C34H44O19 |
| Molecular Weight | 756.71 |
| Solubility | Soluble in Water/DMSO ≥100mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 1592732-453-0 |
| MDL | MFCD03424245 |
| SMILES | OC1=C(O)C=C(/C=C/C(O[C@H]2[C@H](O[C@]3([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@@H](O)[C@H](OCCC4=CC=C(O)C(O)=C4)O[C@@H]2CO[C@H]5[C@H](O)[C@](CO)(O)CO5)=O)C=C1 |
| Target Point | TNF Receptor |
| Passage | Apoptosis |
| Background | It inhibits TNF-alpha, IL-6, IκB, and modulates NF-κB. |
| Biological Activity | Forsythoside B是传统中药植物独一味的叶子中分离的苯乙醇苷。独一味可用于治疗炎症疾病和促进血液循环。Forsythoside B 可抑制 TNF-alpha，IL-6，IκB， 调节 NF-κB。[1-3] |
| In Vitro | 连翘苷B浓度依赖性地下调脂多糖(LPS)刺激的RAW264.7细胞中TNF-α，IL-6和高迁移率族蛋白1(HMGB1)的水平，抑制IκB激酶(IKK)途径和调制核因子(NF)- κB[1]。 |
| In Vivo | 静脉注射单独使用连翘苷B或加亚胺培南可降低血清TNF-α，IL-6，HMGB1水平，触发髓样细胞(TREM-1)和内毒素表达受体，而血清IL-10水平上调，肺，肝和小肠中的髓过氧化物酶(MPO)减少[1]。剂量高于8mg/kg的连翘苷B在脑缺血和再灌注大鼠中产生显著的神经保护作用。即使在脑缺血和再灌注后1小时，3小时和5小时延迟给药，连翘苷B(20mg/kg)也表现出显著的神经保护活性。连翘苷B 20 mg/kg可减轻组织病理学损伤，这可通过较小的脑梗塞面积和脑水肿，脑埃文沙蓝外渗和髓过氧化物酶活性降低，抑制脑磷酸化IκB-α和NF-κB表达来证实[2]。连翘苷B显示心肌功能显著恢复，LVSP和+/- dp/dt(max)改善。心肌梗死体积，血清Tn-T，TNF-α和IL-6水平，MDA含量和心肌组织MPO活性均降低，HMGB1蛋白表达，磷酸化蛋白-κB-α和磷酸化NF-κB下调，同时减少SOD和GPx活性的下降[3]。 |
| Cell Experiment | 将连翘苷B溶解在无菌盐水溶液中，并以各种浓度(0.1至10μM)加入培养基中，并与LPS刺激的RAW264.7细胞一起温育。在连翘酯苷B处理24小时后收集无细胞上清液。通过测量培养基中的乳酸脱氢酶(LDH)来评估细胞活力[1]。 |
| Animal Experiment | 大鼠：将连翘苷B溶解在无菌盐水中。对于剂量 - 反应研究，在再灌注后15分钟，以1.3，3.2，8，20或50mg/kg的剂量施用连翘苷B作为静脉推注。假处理或媒介物处理的大鼠注射盐水。再灌注后23小时测定神经功能缺损，然后进行脑梗塞体积检查[2]。 |
| Data Literature Source | [1]. Jiang WL，et al. Forsythoside B protects against experimental sepsis by modulating inflammatory factors. Phytother Res. 2012 Jul;26(7):981-7. [2]. Jiang WL，et al. Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury. Eur J Pharmacol. 2010 Aug 25;640(1-3):75-81. [3]. Jiang WL，et al. Cardioprotection with forsythoside B in rat myocardial ischemia-reperfusion injury: relation to inflammation response. Phytomedicine. 2010 Jul;17(8-9):635-9 |
| Unit | Bottle |
| Specification | 5mg 10mg 10mM*1mL in DMSO 20mg |
可抑制 TNF-alpha，IL-6，IκB， 调节 NF-κB。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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