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CAS：573-58-0
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Red to reddish brown Solid
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| CAS | 573-58-0 |
| Name | Congo Red |
| Molecular Formula | C32H22N6Na2O6S2 |
| Molecular Weight | 696.66 |
| Solubility | SolubleinWater≥10mg/mL;SolubleinDMSO≥1mg/mL |
| Purity | HPLC≥98% |
| Appearance | Red to reddish brown Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 209-358-4 |
| MDL | MFCD00004028 |
| SMILES | O=S(C1=C2C=CC=CC2=C(N)C(N=NC3=CC=C(C4=CC=C(N=NC5=CC(S(=O)([O-])=O)=C6C=CC=CC6=C5N)C=C4)C=C3)=C1)([O-])=O.[Na+].[Na+] |
| InChIKey | IQFVPQOLBLOTPF-UHFFFAOYSA-L |
| InChI | InChI=1S/C32H24N6O6S2.2Na/c33-31-25-7-3-1-5-23(25)29(45(39,40)41)17-27(31)37-35-21-13-9-19(10-14-21)20-11-15-22(16-12-20)36-38-28-18-30(46(42,43)44)24-6-2-4-8-26(24)32(28)34;;/h1-18H,33-34H2,(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2 |
| PubChem CID | 11313 |
| Background | Congo Red， an anionic diazo dye based on benzidine， has been widely used for the determination of amyloid in tissue sections and as an additive to biological media. |
| Biological Activity | Congo red 是一种偶氮染料。Congo red 结合已被广泛用于组织切片中淀粉样蛋白的测定[1]。 |
| In Vitro | 刚果红组织化学染色可作为一种简单的筛选工具，用于研究突变细胞中的聚集体是否具有错误折叠的β-折叠片状二级结构。刚果红组织化学染料具有与交叉的β-褶皱片结构特异性结合的能力。野生型HSPB1应通过以非天然构象结合蛋白质来维持蛋白质稳态，从而防止底物聚集。然而，T139M突变体在该功能中失败并导致错误折叠的蛋白质的累积，其被刚果红靶向以插入β-折叠的片状结构之间。刚果红组织化学染色可以作为一个简单的工具，以研究突变细胞中的聚集体是否具有错误折叠的β-折叠片二级结构[1]。 |
| Cell Experiment | 由于其大的细胞质体积，选择HeLa细胞用于这些研究。用突变体或野生型HSPB1构建体转染的细胞在盖玻片上生长24小时，然后用刚果红染色以确定聚集体是否显示淀粉样蛋白形成特性。简而言之，首先用10％福尔马林固定细胞10分钟，并用1％刚果红染色5分钟，然后用0.01％氢氧化钾在50％乙醇中脱色。然后将盖玻片通过分级乙醇浓度进行脱水并安装在封固剂中，并在罗丹明过滤器下通过荧光显微镜检查[1]。 |
| Data Literature Source | [1]. Amornvit J，et al. A novel p.T139M mutation in HSPB1 highlighting the phenotypic spectrum in a family. Brain Behav. 2017 Jul 21;7(8):e00774 |
| Unit | Bottle |
| Specification | 100mg 10mM*1mL in Water 250mg |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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