Aconitase（ACO）Activity Assay Kit
Cat.No：BC4485 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Tricarboxylic Acid Cycle Series > Aconitase（ACO）Activity Assay Kit
Product Information
| Name | Aconitase（ACO）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Aconitase (ACO) is an important intracellular ferritin, which is mainly present in the cytoplasm and mitochondria. ACO catalyzes the reversible reaction of intracellular citric acid to isocitric acid via cis aconitic acid, which plays an important role in maintaining the success of the tricarboxylic acid cycle and the glyoxylic acid cycle.
Measurement Principle：
Aconitase can catalyze isocitrate to produce aconitic acid. Cisaconitic acid has a characteristic absorption peak at 240 nm. The enzyme activity is calculated by measuring the rate of cisaconitic acid production.
Self Provided：
Spectrophotometer/microplate reader, desk centrifuge, water bath/constant temperature incubator, adjustable transferpettor, ultrasonic cell pulverizer, micro quartz cuvette/96 well (UV) plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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