{{cart_num}}
My Cart
Application：ELISA
Reactivity：Bovine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：0.09ng/mL
Storage：2-8℃，6 months
Gene ID：280829
Qty：
Size：
| Name | Bovine Insulin ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Insulin |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Bovine |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 0.17-5.5ng/mL |
| Sensitivity | 0.09ng/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | A5PJB2 |
| Gene ID | 280829 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗牛Insulin单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的牛Insulin会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗牛Insulin抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的牛Insulin发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的牛Insulin，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的牛Insulin浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中牛Insulin的浓度。 |
| Background | IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17，000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells，although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species，the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors，designated type I and type II.?Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy，binding IL-1 and thus preventing binding of IL-1 to the type I receptor. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
