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Application： ELISA
Reactivity：Mouse
Sensitivity：9.38 pg/ml
Storage：2-8℃，6 months
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| Name | Mouse BNP ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | BNP |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Competitive-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.63-1000 pg/ml |
| Sensitivity | 9.38 pg/ml |
| Specificity | Can detect Mouse BNP in the sample, and there is no significant crossover with other related proteins. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 1.25h |
| Swiss Prot | P40753 |
| Gene ID | 18158 |
| Detection Principle | Solarbio ELISA试剂盒采用竞争ELISA法。用小鼠BNP抗原包被于酶标板上，实验时样品或标准品中的小鼠BNP与包被的小鼠BNP竞争生物素标记的抗小鼠BNP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠BNP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠BNP的浓度。 |
| Background | Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro?BNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure，while mature BNP (aa 103?134) promotes vasodilation and fluid and sodium excretion. Human BNP precursor shares 29% and 51% aa sequence identity with mouse and porcine BNP precursor，respectively. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Arabidopsis COPT1 copper transporter uses a single histidine to regulate transport activity and protein stability
Click to check >>Author：Wenci Weng, Xuewen Yu, Yifan Dong, Taifen Wang, Mumin Shao, Huili Sun, Pengxun Han
IF：3.4000
Publish_to：Molecular Medicine Reports
PMID：37387406
