Anti-Flag tag Monoclonal Antibody
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Cat.No：K200001M Solarbio
Application： WB,IP,IF
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Mouse
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Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
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Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
Experimental Images
Related Products
Related Conjugation
Related Paper
Technical Q&A
Product Information
| Name | Anti-Flag tag Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 2C5 |
| Dilution Ratio | WB 1:10000-15000. IP 1:100-300. IF 1:500-3000. |
| Target | Flag tag |
| Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence. |
| Immunogen | A synthetic peptide coupled to KLH |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Application | IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Western blot analysis with Flag tag antibody diluted at 1:10000
Related Products
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Click to check >>Author：Liu R
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PMID：35421574
Phosphorylation of SiRAV1 at Ser31 regulates the SiCAT expression to enhance salt tolerance in Setaria italica
Click to check >>Author：Qiao-lu LI, Zhi-yong LI, Meng-meng WANG, Jing-wei YAN, Lin FANG
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Effects of ELL-associated factor 2 on ultraviolet radiation-induced cataract formation in mice
Click to check >>Author：Yanhua Jiang, Rongrong Fu, Jiangyue Zhao, Di Wu, G
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