Ploygalacturonase(PG) Activity Assay Kit
Cat.No：BC2660 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Pectin Series > Ploygalacturonase(PG) Activity Assay Kit
Product Information
| Name | Ploygalacturonase(PG) Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
Polygalacturonase (PG) is a type of pectinase that is widely found in plants, bacteria and fungi. It catalyzes the decomposition of polygalacturonic acid, and plays an important role in softening fruits, pollen pollination, maturity of seeds, and shedding of organs. When pathogenic bacteria infect host plants, they can secrete polygalacturonase to degrade the host the cell wall, in turn, leads to the development of the disease course.
Measurement Principle：
PG hydrolyzes polygalacturonic acid to generate galacturonic acid, and galacturonic acid reacts with DNS reagents to produce a brown-red substance with a characteristic absorption peak at 540 nm. Pectinase activity can be calculated by measuring the change in absorbance at 540 nm.
Self Provided：
Spectrophotometer, centrifuge, water bath, 1 mL glass cuvette, mortar/ homogenizer/cell ultrasonic crusher, transferpettor, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Analysis of seed production and seed shattering in a new artificial grassland forage: pigeon pea.
Click to check >>Author：Xinyong Li, Wei Sheng, Qianzhen Dong, Rui Huang, Rongshu Dong, Guodao Liu, Xipeng Ding, Jingwen Zhang
IF：6.6270
Publish_to：Frontiers in Plant Science
PMID：37251779
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