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My CartReactivity：Fusion proteins containing the HIS Tag
Application： WB
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Host：Rabbit
Qty：
Size：
| Name | Anti-6×His Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-10000. |
| Target | 6×His |
| Background | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
| Immunogen | A synthetic peptide |
| Calculated MW | 49/45kDa |
| Purification Method | Affinity purification |
| Storage Buffer | pH7.4 PBS，0.05% NaN3，40% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Fusion proteins containing the HIS Tag |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with 6��His antibody diluted at 1:5000; Lane 1-5: Recombinant protein
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Amide-containing neoepitopes: the key factor in the preparation of hapten-specific antibodies and a strategy to overcome
Click to check >>Author：Chen-Chen Lyu, Bao Yuan, Hao-Yu Che, Yu Meng, Yi Zheng, Yun-Tong He, Zhong-Hao Ji, Shuai Cong, Xing-Yu Ji, Hao Jiang, Jia-Bao Zhang
IF：8.2000
Publish_to：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
PMID：37315671
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