Free Cholestenone(FC) Content Assay Kit
Cat.No：BC1890 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Fatty Acid Metabolism Series > Free Cholestenone(FC) Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Free Cholestenone(FC) Content Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
FC is the main component of cell membrane, and it is also an important raw material for the synthesis of adrenocortical hormone, sex hormone, bile acid and vitamin D. The concentration of FC can be used as an index of lipid metabolism.
Measurement Principle：
The determination principle: FC oxidase catalyzes FC to form 4-cholesterolenone and H2O2, while the peroxidase catalyzes H2O2, 4-aminoantipyrine and phenol to form red quinone compounds with an absorption peak at 500 nm, and the color depth is proportional to the content of FC.
Self Provided：
Water bath, pipettes, spectrophotometer, 1 mL glass cuvette, mortar/homogenizer/cell ultrasonic crusher, absolute ethanol, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Free Cholestenone(FC) Content Assay Kit
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