Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit
Cat.No：BC1150 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > REDOX Series > Glutathione Series > Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
Thioredoxin Reductase (TrxR) is a NADPH-dependent dimer selenase and includes FAD structure domain. TrxR belongs to pyridine nucleotide-disulfide REDOX enzyme, and form thioredoxin system with thioredoxin and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduct to GSH, which is the key enzyme in glutathione REDOX cycle.
Measurement Principle：
TrxR catalyzes NADPH to reductDTNB form TNB and NADP+, TNB has a absorbance at 412 nm, but reduced glutathione reacts with DTNB to form TNB, so the 2-Vinylpyridine in this kit can inhibit reduced glutathione in sample. The activity of TrxR can be calculated by detecting increase rate of TNB at 412 nm.
Self Provided：
Spectrophotometer, low temperature centrifuge, adjustable pipette, 1 mL glass cuvette and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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