Dehydroascorbate Reductase(DHAR) Activity Assay Kit
Cat.No：BC0660 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > REDOX Series > Vitamin C Metabolism Series > Dehydroascorbate Reductase(DHAR) Activity Assay Kit
Product Information
| Name | Dehydroascorbate Reductase(DHAR) Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
ehydroascorbate reductase (DHAR) is an important antioxidant enzyme in plants and a key enzyme that promotes ascorbic acid regeneration in the ascorbate-glutathione oxidation cycle. In the circulation DHAR maintain the normal metabolic level of ascorbic acid in plants through ascorbic acid, and plays an important role in protecting cellular components from oxidative damage.
Measurement Principle：
DHAR catalyzes the reduction of dehydroascorbic acid (DHA) by reducing glutathione (GSH) to produce AsA. GSH can react with 5,5'-dithio-bis- (2-nitrobenzoic acid) (DTNB) to produce 2- Nitro-5-mercaptobenzoic acid (TNB) and glutathione disulfide (GSSG). TNB has maximum light absorption at a wavelength of 412 nm. DHAR activity is calculated by measuring the reduction rate of GSH.
Self Provided：
Low temperature centrifuge, spectrophotometer, water bath, mortar/homogenizer, 1 mL glass cuvette, adjustable pipette, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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