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My Cart> Antibodies > Loading Control Antibodies > Mitochondria > Anti-COX4I1 Polyclonal AntibodyReactivity：Mouse,Human,Rat
Application： IP,IF,WB,IHC
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-COX4I1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-5000. IHC 1:50-200. IF 1:50-200. IP 0.5μg-4μg. |
| Target | COX4I1 |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Swiss Prot | P13073 |
| Gene ID | 1327 |
| Subcellular Location | Cytoplasm Mitochondrion inner membrane |
| Immunogen | Recombinant protein of human COX4I1 |
| Public Immunogen Range | 23-98aa |
| Calculated MW | 20kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human Rat |
| Application | IP IF WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with COX4I1 antibody diluted at 1:1000;Lane: 293T Mouse liver Rat brain.
Immunohistochemistry of paraffin-embedded breast cancer��hu�� using COX4I1 antibody at dilution of 1:100 (x100 lens,x200 lens).
Immunofluorescence analysis of HeLa cell using COX4I1 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using COX4I1 antibody diluted at 1:100.
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