Serum Free Medium for Mesenchymal Stem Cells

Product Description:

Stem cell serum-free medium is a product optimized by using the latest formula and repeated trials. It avoids the shortcomings of the traditional medium containing fetal bovine serum, such as the introduction of foreign components and unclear ingredients, and contains a series of components that can replace fetal bovine serum: Essential and non-essential amino acids, vitamins, hormones, growth factors, organic and inorganic compounds and other supplements. The production process is controlled in strict accordance with GMP standards to ensure the quality of products.

Operation instructions:

1. One hour before the start of the experiment, the ingredients without serum additives were dissolved at 2-8℃. After the dissolution is complete, gently shake the reagent bottle to thoroughly mix the freeze-thaw ingredients.

2. Use 75% alcohol to disinfect the open outer wall of each bottle in the kit.

3. After the alcohol on the reagent bottle is volatilized, open each reagent bottle aseptically.

4. The additive was added to serum-free mesenchymal stem (matrix) cell base medium.

5. Aseptically absorb the base medium washing additive bottle, and add all the ingredients of the additive into the base medium as completely as possible.

6. Repeat the steps two to three times.

7. Gently shake the base medium bottle to mix the ingredients well. The prepared complete medium was placed at 4℃ for storage.

Note: The company's products are sterile, but to ensure sterility, it is recommended that after all reagents are mixed, the complete medium is filtered again for sterilization (0.22μm).

Scope of application :

The product is suitable for the growth of human mesenchymal stem cells from multiple sources, such as umbilical cord (UCM-hMSC), bone marrow (BM-hMSC), adipose tissue (AT-hMSC), etc.

Product Features :

1, comprehensive nutrition, safe use;

2, the growth rate was fast, the phenotype was obvious, the cell state was good, and the culture did not differentiate until 10 generations;

3, can support the primary isolation and culture of bone marrow, umbilical cord, fat and other tissue derived mesenchymal stem cells;

4, can maintain the potential of stem cells to differentiate into adipocytes, osteoblasts and chondrocytes;

5, in accordance with strict GMP requirements for production, endotoxin content is less than 0.25EU/ml;

6, stable performance, cost-effective.

Note:

1, if there is more visible sediment in the medium, it should not be used again;

2, exceed the expiration date on the product label, shall not be used again;

3, this product is only suitable for scientific research;

4, this product should be stored in a cool and dry place away from light, the storage conditions of basic medium: 2-8℃, the storage conditions of additives -20℃, should avoid repeated freezing and thawing;

5, after the preparation of complete media, as soon as possible within 2 weeks to use.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.