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My Cart> Antibodies > Loading Control Antibodies > Nucleus > Anti-HDAC1 Polyclonal AntibodyReactivity：Rat,Human,Mouse
Application： WB,IF,IHC,IP
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-HDAC1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:50-100. IP 0.5μg-4μg. |
| Target | HDAC1 |
| Background | Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. |
| Swiss Prot | Q13547 |
| Gene ID | 3065 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human HDAC1 |
| Public Immunogen Range | 393-482aa |
| Calculated MW | 55kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded breast cancer��hu��using HDAC1 antibody at dilution of 1:100 (x100 lens��x200 lens).
Immunofluorescence analysis of A549 cell using HDAC1 antibody. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody.
Western blot analysis with HDAC1 antibody diluted at 1:1000;Lane: 293T HeLa HepG2 MCF7 Mouse thymus Mouse testis Rat thymus Rat testis.
Immunohistochemistry of paraffin-embedded rat spleen with HDAC1 antibody diluted at 1:150.
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