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My Cart> Antibodies > Loading Control Antibodies > Nuclear Membrane > Anti-LMNA Monoclonal AntibodyReactivity：Human,Rat,Mouse
Application： ICC,IF,WB
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Host：Mouse
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| Name | Anti-LMNA Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 5D12-C6-E9 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. ICC 1:50-200. |
| Target | LMNA |
| Background | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. |
| Swiss Prot | P02545 |
| Gene ID | 4000 |
| Subcellular Location | Nucleus Nucleus envelope Nucleus lamina Nucleus speckle nucleoplasm |
| Immunogen | Recombinant protein of human LMNA |
| Calculated MW | 74/65/71/64/63/69kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | ICC IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with LMNA antibody diluted at 1:1000;Lane: MCF-7 A549 HeLa.
Immunofluorescence analysis of A549 using LMNA antibody diluted at 1:100.
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